Features and Benefits

Compatible with high-throughput handling systems.

General description

Aconitase (Aconitate Hydratase) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe⁴S⁴] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich′s ataxia and diabetes.

Principle

The Aconitase Assay Kit measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.

Suitability

Suitable for aconitase activity determination in biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)